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recombinant proteins ghost dyetm red 780 tonbo biosciences  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences recombinant proteins ghost dyetm red 780 tonbo biosciences
    Recombinant Proteins Ghost Dyetm Red 780 Tonbo Biosciences, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ghost+dye+red+780/Ghost+Dye+Red+780/pm41923619-215-77-83
    Average 96 stars, based on 507 article reviews
    recombinant proteins ghost dyetm red 780 tonbo biosciences - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: Tabular foundation model predicts alternative lengthening of telomeres (ALT) and identifies SMARCAL1 as a target in ALT-driven cancers
    Article Snippet: Proliferation assays were run and analyzed by an IncuCyte S3 instrument (Sartorius, Gottingen, Germany.)

    Article Title: A post-translational regulatory map of chronic antigen-driven human T cell dysfunction
    Article Snippet: Spleens and tumors were then cut in small pieces and incubated for 45 min at 37 °C, 250 rpm shaking, in 1-2 mL of digestion buffer containing RPMI-1640 medium supplemented with 100X DNase I (Millipore Sigma, 10104159001; final concentration: 0.1 mg/mL), 350 units/mL hyaluronidase (Worthington Biochemical Corporation, LS002592) and 50 μg/mL Liberase (Millipore Sigma, 05401020001).

    Staining:

    Article Title: A lipid nanoparticle-based mRNA vaccine elicits immunity against porcine circovirus type 2 in mice.
    Article Snippet: .. The cells were first stained with Ghost Dye Red 780 (TONBO Biosciences, Cat. . 13-0865-T100) for dead cells and then stained with a cocktail of the following fluorescently labeled antibodies: anti-CD45-Alexa Fluor 700 (BioLegend, Cat. 157210), anti-CD4-FITC (BioLegend, Cat. 100509), anti-CD185-Brilliant Violet 605 (BioLegend, Cat. 145513), anti-PD-1-Brilliant Violet 421 (BioLegend, Cat. 135221), anti-B220-PerCP-Cya nine5.5 (BioLegend, Cat. 103236), anti-CD95-PE (BioLegend, Cat. 152608), and anti-GL-7APC (BioLegend, Cat. 144618) in cell staining buffer and incubated for 20 min in the dark at room temperature. .. Data for all samples were acquired on a BD FACSymphony flow cytometer (BD Biosciences) and analyzed using FlowJo software (v10.8).

    Article Title: Aminosteroid RM-581 Induces G0/G1 Arrest and Endoplasmic Reticulum Stress-Mediated Apoptosis in Human Acute and Chronic Leukemia Cell Lines
    Article Snippet: .. The cells were stained first with Ghost Dye Red 780 (0.05 μL per 1 × 105 cells) (Cytek Biosciences Inc., Fremont, CA, USA) according to the manufacturer’s instructions. .. The cells were then washed and stained with anti-CD19-FITC (2.5 μL per 1 × 105 cells), anti-CD3-PE (2.5 μL per 1 × 105 cells), anti-CD4-PE/Cy7 (1 μL per 1 × 105 cells) and anti-CD8-PerCP (1 μL per 1 × 105 cells) (Biolegend Inc., San Diego, CA, USA) and incubated on ice for 20 min in the dark.

    Article Title: GPR182 is a lipoprotein receptor for dietary fat absorption
    Article Snippet: .. The main staining antibodies 27 used in this study are as follows: anti-human GPR182 (Catalog # FAB10293R, R&D Systems); anti-human LDLR (Catalog # 565653, BD biosciences); anti-mouse LDLR (Catalog # FAB2255P, R&D Systems); anti-human SR-B1 (Catalog # 363203, BioLegend); anti-mouse SR-B1 (Catalog # 163102, BioLegend); anti-mouse CD45 (Catalog # 103122, BioLegend); anti-mouse CD146 (Catalog # 134704, BioLegend); Ghost Dye® Red 780 (Catalog # 13-0865-T100, CYTEK); antimouse CD45 (Catalog # 103155, BioLegend); anti-mouse CD31 (Catalog # 102406, BioLegend); anti-mouse Lyve1 (Catalog # FAB2125P, R&D Systems). .. Data were acquired on CytoFLEX and analyzed using FlowJo 11 software.

    Article Title: Targeting TPX2-dependent lineage plasticity by CDK4/6 inhibition reverses therapy resistance in neuroendocrine bladder carcinoma.
    Article Snippet: .. After the coculture period, the cells were collected and stained with Ghost Dye Red 780 (Tonbo Biosciences, Cat13-0865-T500) for viability, followed by staining with antibodies specific for CD8 (BioLegend, Cat#980908, RRID:AB_2888883), PD-1 (BioLegend, Cat#379209, RRID:AB_2922607), and TIM-3 (BioLegend, Cat#364819, RRID:AB_3683393). ..

    Article Title: A post-translational regulatory map of chronic antigen-driven human T cell dysfunction
    Article Snippet: Cells were then washed with 9 volumes of 1 X PBS to remove ACK buffer, centrifuged at 500 x g for 5 min at 4 °C, and proceeded with Dynabeads Untouched Mouse CD8 Cells Kit (ThermoFisher, 11417D) following manufacturer’s instruction to enrich for CD8 + T cells. .. After enrichment, cells were stained on ice for viability staining with 1:2000 Ghost Dye Red 780 (Cytek Biosciences, 13-0865-T100) and 1:100 TruStain FcX (BioLegend, 101320) in 1X PBS for 10 min. ..

    Labeling:

    Article Title: A lipid nanoparticle-based mRNA vaccine elicits immunity against porcine circovirus type 2 in mice.
    Article Snippet: .. The cells were first stained with Ghost Dye Red 780 (TONBO Biosciences, Cat. . 13-0865-T100) for dead cells and then stained with a cocktail of the following fluorescently labeled antibodies: anti-CD45-Alexa Fluor 700 (BioLegend, Cat. 157210), anti-CD4-FITC (BioLegend, Cat. 100509), anti-CD185-Brilliant Violet 605 (BioLegend, Cat. 145513), anti-PD-1-Brilliant Violet 421 (BioLegend, Cat. 135221), anti-B220-PerCP-Cya nine5.5 (BioLegend, Cat. 103236), anti-CD95-PE (BioLegend, Cat. 152608), and anti-GL-7APC (BioLegend, Cat. 144618) in cell staining buffer and incubated for 20 min in the dark at room temperature. .. Data for all samples were acquired on a BD FACSymphony flow cytometer (BD Biosciences) and analyzed using FlowJo software (v10.8).

    Incubation:

    Article Title: A lipid nanoparticle-based mRNA vaccine elicits immunity against porcine circovirus type 2 in mice.
    Article Snippet: .. The cells were first stained with Ghost Dye Red 780 (TONBO Biosciences, Cat. . 13-0865-T100) for dead cells and then stained with a cocktail of the following fluorescently labeled antibodies: anti-CD45-Alexa Fluor 700 (BioLegend, Cat. 157210), anti-CD4-FITC (BioLegend, Cat. 100509), anti-CD185-Brilliant Violet 605 (BioLegend, Cat. 145513), anti-PD-1-Brilliant Violet 421 (BioLegend, Cat. 135221), anti-B220-PerCP-Cya nine5.5 (BioLegend, Cat. 103236), anti-CD95-PE (BioLegend, Cat. 152608), and anti-GL-7APC (BioLegend, Cat. 144618) in cell staining buffer and incubated for 20 min in the dark at room temperature. .. Data for all samples were acquired on a BD FACSymphony flow cytometer (BD Biosciences) and analyzed using FlowJo software (v10.8).

    Article Title: Uncovering a new player in ischemic stroke: a study of intra-arterial interferon-gamma-producing CD14 + myeloid cells in hyperacute stroke.
    Article Snippet: .. First, viable vs nonviable cells were distinguished by washing the cells twice with phosphate-buffered saline (PBS), followed by incubation with Ghost Dye Red 780 (Cat. 13-0865-T100, Tonbo Bioscience) for 30 minutes at 2 ̊C. .. The cells were subsequently washed twice with FACS buffer consisting of 1X Dulbecco’s phosphate-buffered saline (PBS) (Cat. 21-0310CV, Corning, NY), 1% bovine serum albumin (Cat. BP1600-100, Fisher Scientific, NH), and 0.1% sodium azide (Cat. S2002, Millipore Sigma, MA).

    Saline:

    Article Title: Uncovering a new player in ischemic stroke: a study of intra-arterial interferon-gamma-producing CD14 + myeloid cells in hyperacute stroke.
    Article Snippet: .. First, viable vs nonviable cells were distinguished by washing the cells twice with phosphate-buffered saline (PBS), followed by incubation with Ghost Dye Red 780 (Cat. 13-0865-T100, Tonbo Bioscience) for 30 minutes at 2 ̊C. .. The cells were subsequently washed twice with FACS buffer consisting of 1X Dulbecco’s phosphate-buffered saline (PBS) (Cat. 21-0310CV, Corning, NY), 1% bovine serum albumin (Cat. BP1600-100, Fisher Scientific, NH), and 0.1% sodium azide (Cat. S2002, Millipore Sigma, MA).



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